Determination of Main Components in Minocycline Hydrochloride Sustained-release Tablets by HPLC
- VernacularTitle:HPLC法测定盐酸米诺环素缓释片主药的含量
- Author:
Jing QI
;
Linhua WU
- Publication Type:Journal Article
- Keywords:
Minocycline hydrochloride;
HPLC;
Sustained-release tablets;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for determining the content of main components in minocycline hydrochloride sustained-release tablets.METHODS:The sample was separated on ODS-C_(18)column with the mobile phase consisted of 0.2 mol?L~(-1)ammonium oxalate-0.01 mol?L~(-1)edetate disodium-dimethylformamide-tetrahydrofuran (600:180:120:80)at a flow rate of 1.5 mL?min~(-1).The detection wavelength was set at 280 nm and the sample volume was 20?L.RESULTS:The linear range of minocycline was 0.05～0.5 mg?mL~(-1)(r=0.999 8)and the average recovery was 99.11%,RSD=0.5%.CONCLUSIONS:This method was proved to be accurate and reliable,sensitive and reproducible,and it can be used for determining the content of this tablets.
