Content Determination of Chlorhexidine Acetate in Daobian Suppository by RP-HPLC
- VernacularTitle:RP-HPLC法测定导便栓中醋酸氯己定的含量
- Author:
Qiuhong CHEN
;
Jiying YU
;
Jin CHEN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Chlorhexidine acetate;
Daobian suppository;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of the content of chlorhexidine acetate in daobian suppository.METHODS:The determination was performed on Diamonsil C_(18)column,The mobile phase consisted of acetonitrile-water-triethylamine(30:70:0.5,with pH adjusted to 3.0 by phosphoric acid)at a flow rate of 1.0 mL?min~(-1),The detective wavelength was 260 nm,the sample size was 20?L and the column temperature was maintained at 30℃. RESULTS:The linear range of Chlorhexidine Acetate was 1.092～13.10?g?mL~(-1)(r=0.999 9)and its average recovery was 98.29%(RSD=2.16%,n=9).CONCLUSIONS:The method is simple and accurate,and it can be used for the determination of the content of chlorhexidine acetate in daobian suppository.
