Effects of Different Extraction Methods on the Content of Cholalic Acid from Artificial Bezoar
- VernacularTitle:不同提取方法对人工牛黄中胆酸含量的影响
- Author:
Zhenzhen XU
;
Yiping HUANG
- Publication Type:Journal Article
- Keywords:
Artificial Bezoar;
Cholalic acid;
Hydrolyzes;
HPLC-ELSD;
Content
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of different extraction methods on the content of cholalic acid from Artificial Bezoar. METHODS: Artificial Bezoar from 3 different habitats was used to extract cholalic acid by hydrolization vs. hydrolization-free method, and the content of cholalic acid was determined by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). RESULTS: The content of cholalic acid in the hydrolyzed sample was higher than that in the non-hydrolyzed sample of the same batch. CONCLUSION: Through hydrolization, the binding cholalic acid in the artificial bezoar was transformed into free cholalic acid so that the content of cholalic acid was increased. Therefore, it is more reasonable to control the quality of Artificial Bezoar by taking the contents of total cholalic acid and free cholalic acid as indexes.
