Determination of Cholalic Acid and Hyodeoxycholic Acid in Qingkailing Suppository by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定清开灵栓中胆酸和猪去氧胆酸的含量
- Author:
Zhihong XIE
;
Tao ZHOU
;
Xiaoling WANG
;
Ling CHEN
;
Chaofeng LIANG
- Publication Type:Journal Article
- Keywords:
HPLC-ELSD;
Qingkailing suppository;
Cholalic acid;
Hyodeoxycholic acid;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the contents of cholalic acid and hyodeoxycholic acid in Qingkailing suppository by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection). METHODS: The analytical column was Diamonsil C18(250 mm?4.6 mm, 5 ?m) and the mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution) with the temperature of the drift tube of the light scattering detector set at 110 ℃ and the flow rate of the nitrogen gas at 2.0 L?min-1. RESULTS: The linear ranges of cholalic acid and hyodeoxycholic acid were 0.59～7.4 ?g(r=0.999 6)and 0.38～4.8 ?g(r=0.999 7),respectively,with their average recoveries at 99.20%(RSD=1.4%,n=6)and 98.57%(RSD=1.8%,n=6), respectively. CONCLUSION: The method is simple, accurate and well-separated, and it is applicable for the quality control of Qingkailing suppository.
