A study on cells proliferation and invasiveness of Hep-2 inhibited by RNA interference mediated PIK3CA gene silencing

VernacularTitle:RNA干扰介导的PIK3CA基因沉默抑制Hep-2细胞增殖和侵袭力
Author: Shengcai WANG ; Jugao FANG ; Xin NI ; Zhigang HUANG ; Qi WANG ; Xiaohong CHEN ; Wei ZHANG ; Qi ZHONG ; Hong WANG ; Lingzhao MENG
Publication Type:Journal Article
Keywords: Laryngeal Neoplasms; Cell Proliferat- ion; Neoplasm Invasiveness; 1-Phosphatidylinositol 3-Kinases; RNA Interference
From: Chinese Archives of Otolaryngology-Head and Neck Surgery 2006;0(03):-
CountryChina
Language:Chinese
Abstract: OBJECTIVE To observe the effects of RNA interference mediated PIK3CA gene silencing on the proliferation and invasiveness of laryngeal squamous cell carcinoma(LSCC) cells, and investigate the feasibility of PIK3CA gene as a potential therapeutic target in the treatment of LSCC. METHODS The lentiviral vector system expressing short hairpin RNA targeting PIK3CA gene(PIK3C-shRNA)was constructed and transfected subsequently into Hep-2 cells mediated by liposome in vitro. The expression of PIK3CA gene was detected by real-time RT-PCR and Western blot respectively. The proliferation of Hep-2 cells was measured by MTT, colony formation, and cell growth curve. The invasive power was determined by Boyden chamber model in vitro. RESULTS The lentiviral vector system expressing short hairpin PIK3CA- shRNA was constructed successfully. Compared with the control groups, the mRNA and protein expression of PIK3CA were significantly down-regulated(75% and 70% respectively)in the experimental group (P