Caspase-3 expression in the gentamycin-induced apoptosis of guinea pig hair cells
- VernacularTitle:庆大霉素致豚鼠耳蜗毛细胞凋亡时Caspase-3的表达
- Author:
Yunpeng MA
;
Xuejun JIANG
;
Jimin BAO
- Publication Type:Journal Article
- Keywords:
Guinea Pigs;
Gentamicins;
Apoptosis;
Caspases
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To discuss the gentamycin-induced ototoxity and mechanism on cochlear outer hair cells. METHODS All the guinea pigs were randomly divided into 3 groups. Gentamycin, gentimycin plus furosemide and normal saline were intraperitoneally injected for 6 days respectively. The amplititude of distortion product otoacoustic emissions (DPOAE)urface prepration of cochlear basilar membrane, hair cells’ apoptosis and expression of caspase-3 after injection were analyzed. RESULTS Reduction of the DPOAE amplitude, hair cell damage, apoptosis and Caspase-3 expression were observed in group injected with gentamycin as well as in group injected with gentamycin plus furosemide. CONCLUSION Gentamycin should induce hair cell damage in the cochlea of guinea pigs and Caspase-3 plays an important role in the process of hair cell apoptosis.
