Low Molecular Weight Heparin's Interference with Immunocytes' Adhesion to Fibroblast-like Synovocyte (FLS) from Patients with Rheumatoid Arthritis(RA):An in Vitro Study
- VernacularTitle:低分子量肝素干扰免疫细胞黏附类风湿性关节炎滑膜细胞的体外研究
- Author:
Lianbo XIAO
;
Guilin OUYANG
;
Xinwen ZHANG
;
Yong HE
;
Zheng HUANG
;
Yongli ZHANG
;
Jun XIE
- Publication Type:Journal Article
- Keywords:
Low molecular weight heparin;
CYR61;
Rheumatoid arthritis;
Fibroblast-like synovocytes;
Adhesion
- From:
China Pharmacy
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effect and thepossible mechanism of low molecular weight heparin(LMWH)on immunocytes' adhesion to fibroblast-like synovocytes(FLS)isolated from patients with rheumatoid arthritis(RA)and to study LMWH'S possible anti-inflammatory effect on RA.METHODS:LMWH's interference on the adhesion of peripheral blood monouclear cells(PBMC)isolated from healthy volunteers to FLS of RA patients was determined by quantitative counting using flow cytometry.The expression of CYR61 in the in vitro cultured FLS of RA patients was detected using real-time PCR technology.RESULTS:When FLS culture system was added with PBMC,PBMC were obviously found to adhere to FLS,but the number of adhered PBMC decreased after LMWH treatment,which manifested as increase of deciduous PBMC,increased more with the increase of LMWH dose.There was a high expression of CYR61 in synovium tissue in RA patients.CONCLUSION:LMWH inhibited the adheresion of PBMC to FLS from RA patients in a dose-dependent manner,which might be attributed to its competitive combination with heparin sulfate sites on CYR61.
