Effect of the Serum Contained Radix Astragali and Hirudo and Their Compound on the Growth Cycle and Apoptosis of Rat Glomerular Mesangial Cells
- VernacularTitle:黄芪、水蛭及其配方含药血清对大鼠系膜细胞生长周期及凋亡的影响
- Author:
Xianzhi REN
;
Shumin JIANG
;
Wensheng ZHAI
- Publication Type:Journal Article
- Keywords:
Rat;
Mesangial cells (MC);
Growth cycle;
Apoptosis;
Radix Astragali;
Hirudo;
Medicinal serum
- From:
Journal of Traditional Chinese Medicine
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of the serum contained Radix Astragali, Hirudo or their compound on the growth cycle and apoptosis of rat mesangial cells (MC).Methods The serum contained the extract of high-concentration of Hirudo, the serum contained the extract of high-concentration of Radix Astragali, and the serum contained the extract of high-concentration of Hirudo and the extract of low-concentration of Radix Astragali were prepared. The cultured MC were measured by flow cytometer to observe the changes in their growth cycle and apoptosis. The morphological changes of apoptotic cells were observed.Results The medicinal serums can keep the most MC in the stage of G0/G1, and the significant difference was existing in comparison with that of the control group (P
