Assessment of affecting factors in measuring activating platelets with the method of flow cytometry
- VernacularTitle:流式细胞术检测血小板活化的影响因素分析
- Author:
Qingmei LI
;
Jianrui WEI
;
Jiemin HONG
;
Hui CHEN
;
Zhuangbo GUO
;
Biao LI
- Publication Type:Journal Article
- Keywords:
Flow cytometry;
Blood platelets;
Platelet glycoprotein GPⅡb-Ⅲa complex;
CD62P
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the affecting factors of detecting platelet activation by flow cytometry (FCM). METHODS:Using decoagulant of natrium citricum,anticoagnlated peripheral venous bloods from 6 healthy donors were labeled with the method of three-colour immunofluorescence assay. Platelet activation markers fibrinogen receptor (Fib-R,PAC-1) and P-selectin (CD62P) were measured. In the same time,the reproducibility of FCM was assessed.RESULTS:The platelet activation markers PAC-1 and CD62P at each time point showed significant difference(P
