Changes of the expression of CD27 and CD28 on cytokine-induced killer cells and natural killer cells during four week cultivation
- VernacularTitle:CIK/NK细胞4周培养过程中的CD27、CD28的表达及其意义
- Author:
Yanfeng WU
;
Yang LI
;
Xiaoping WANG
;
Jianpei FANG
;
Jing WEI
- Publication Type:Journal Article
- Keywords:
Cytokine-induced killer cells;
Natural killer cells;
CD27;
CD28;
Adoptive immunotherapy
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the changes of CD27 and CD28 expression on cytokine-induced killer(CIK) cells and natural killer(NK) cells during cultivation.METHODS:The mononuclear cells were treated with interleukin-12(IL-2),IL-7,IL-15,stem cell factor and FLT3 ligand for four weeks. The CD27 and CD28 expression on CD3+CD56+ CIK cells and CD3-CD56+ NK cells were examined by flow cytometric analysis every week during four-week cultivation.RESULTS:The expression of CD27 on CIK and NK cells two weeks after cultivation reached the peak value at(44.57?4.07)% and(51.02?5.20)%,respectively. Then,their expression declined gradually to about 30% in the fourth week. The expression of CD28 on CIK and NK cells reached the peak at the third and the second week,the values were (4.40?0.66)% and (45.14?3.58)%,respectively,decreased rapidly and then were almost completely lost at the fourth week. CONCLUSION:According to the changes of expressions of CD27 and CD28 on CIK/NK cells,optimal harvest time of cultured CIK/NK cells should be at the third week of cultivation.
