Regulation of chloride/bicarbonate exchanger on staurosporine-induced mouse cardiomyocyte apoptosis
- VernacularTitle:Cl~-/HCO_3~-交换器对星状孢子素诱导的小鼠心肌细胞凋亡的调节
- Author:
Xiaoming WANG
;
Anheng LIU
;
Yanan CAO
;
Weiwei ZHANG
;
Tangwang SHI
;
Yan LIU
- Publication Type:Journal Article
- Keywords:
Cardiomyocytes;
Apoptosis;
Staurosporine;
Cl-/ HCO-3 exchanger
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate whether the chloride/bicarbonate (Cl-/HCO-3) exchanger is involved in staurosporine (STS) induced mouse cardiomyocyte apoptosis,and further understand the function of Cl-/HCO-3 exchanger in cell apoptosis. METHODS:To induce mouse cardiomyocyte apoptosis and to explore the effect of Cl-/HCO-3 exchanger,STS was used with two methods:⑴ different blocker,the chloride channel as well as exchanger blocker DIDS and the chloride channel blocker NPPB; ⑵ different culture medium with or without HCO-3 component. RESULTS:The cell viability and caspase-3 activity of DIDS and NPPB on STS-induced cardiomyocyte apoptosis were 59.7% and 47.2%,175.0% and 212.0% respectively with significant statistic differences (both P0.05,n=20). The cell viability and caspase-3 activity of STS-induced cardiomyocyte apoptosis in culture medium with or without HCO-3 were 29.8% and 41.6%,553.4% and 424.7%,respectively (both P
