Effects and Mechanisms of Pioglitazone on Expression of Glucose Transporter-4 in Cardiac Muscle Tissue of Rats with Diabetes Mellitus
- VernacularTitle:吡格列酮对糖尿病大鼠心肌葡萄糖转运蛋白-4表达的影响及机制探讨
- Author:
Liping ZHANG
- Publication Type:Journal Article
- Keywords:
Pioglitazone;
Diabetes mellitus;
Rat;
Cardiac muscle;
Glucose transporter-4;
AMP-activated protein kinase
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To observe the effects of long-term administration of pioglitazone at different doses on the expression of glucose transporter-4(GLUT-4) of cardiac muscle tissue in rats and to discuss the possible mechanisms.METHODS: A total of 40 Wistar rats were enrolled,of which,10 were randomly assigned to normal control group(NC),and the other 30 were injected intraperitoneally with streptozotocin to establish diabetes mellitus(DM) rat model.After successful modeling,the rats were assigned to DM model group(DM,normal saline),high and low dose pioglitazone group(HP,LP;0.75,0.25 mg?kg-1?d-1).The NC groups received normal saline for 12 weeks.Blood glucose(BG) level before grouping(baseline level) and prior to death was determined by tail-cutting method.GLUT-4 level in cardiac muscle tissue and both protein and mRNA expression of AMPK? were determined by RT-PCR and western blot,respectively.RESULTS: As compared with NC group,the BG level in DM group was significantly increased but the expression of GLUT-4 and AMPK? decreased significantly(P
