Inhibitory Effect of K_6[P_2W_(18)O_(62)]?14H_2O on Human Breast Tumor Cell MCF-7 Growth in Vitro
- VernacularTitle:K_6[P_2W_(18)O_(62)]?14H_2O抑制人乳腺癌细胞MCF-7生长的体外研究
- Author:
Wenxiu ZHAO
;
Yan LI
;
Shunfu DONG
- Publication Type:Journal Article
- Keywords:
K_6[P_2W_(18)O_(62)]?14H_2O;
Human breast tumor cell MCF-7;
Anti-tumor;
In vitro study
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the in vitro anti-tumor activity of tungstophosphate compound(K6[P2W18O62]?14H2O) with Dawson structure.METHODS: MCF-7 cells were treated with different concentration of K6[P2W18O62]?14H2O,then its IC50 was measured by MTT assay;flow cytometry was applied to analyze cell cycle and apoptosis by computing the ratio of MCF-7 cells in different phase and its apoptotic ratio.Cell growth and viability were measured by trypan blue staining and cell counting by computing the ratio of living cells.The synergetic anti-tumor activity of tungstophosphate compound(K6[P2W18O62]?14H2O) and pharmorubicin was studied.RESULTS: K6[P2W18O62]?14H2O inhibited MCF-7 cell proliferation with IC50 of(33.7?3.2) ?mol?L-1,it reduced the proportions of MCF-7 cells at S phase and G1 phase,with the apoptotic rate at 3.7%～29.2% within 48 h.The percentage of living cells was 95.37%～76.78% at 48 h.As compared with pharmorubicin alone,the addition of K6[P2W18O62]?14H2O to pharmorubicin showed a significantly higher inhibitory action on proliferation of MCF-7 cells(P
