Content Determination of Glucose in Levofloxacin Mesylate/Glucose Injection by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定甲磺酸左氧氟沙星葡萄糖注射液中葡萄糖的含量
- Author:
Wei LI
- Publication Type:Journal Article
- Keywords:
HPLC-ELSD;
Levofloxacin mesylate/glucose injection;
Glucose;
Content determination
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC-ELSD method for the determination of glucose in levofloxacin mesylate/glucose injection(LMGI).METHODS: The samples were determined by HPLC-ELSD on shim-pack VP-ODS column using ammonium acetate solution(adjusted to pH4.0 with acetic acid glacial)-acetonitrile(gradient elution) as the mobile phase at a flow rate of 0.7 mL?min-1.The injection volume was 10 ?L.The ELSD conditions were as follows: pressure of N2=35 PSI;drift temperature=80 ℃;spray temperature=48 ℃;attenuation Gain=10.RESULTS: The linear range of glucose was 49～148 ?g(r=0.999 8) and the average recovery rate was 100.18%(RSD=0.61%).CONCLUSIONS: The method is accurate,specific and applicable for the determination of glucose in LMGI.
