Determination of Amphotericin B in Vaginal Effervescent Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定两性霉素B阴道泡腾片中主药的含量
- Author:
Zhanrui ZHANG
;
Ying XIONG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Amphotericin B;
Vaginal effervescent tablet;
Content determination
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for the content determination of amphotericin B in amphotericin B vaginal effervescent tablets.METHODS: HPLC was performed on Kromasil C18 column with the mobile phase consisted of 0.01 mol ?L-1 potassium dihydrogen-acetonitrile(66∶34) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 408 nm;the sample size was 10 ?L,and the column temperature was set at room temperature.RESULTS: The linearity of amphotericin B was good in the range of 0.404～3.636 ?g(r=0.999 4) and the average recovery rate was 99.39%(RSD=0.22%,n=6).CONCLUSIONS: The method is simple,accurate,feasible,and it can be used for the determination of this tablets.
