Determination of Curcumine in Rujiexiao Tablets by HPLC
- VernacularTitle:HPLC法测定乳结消片中姜黄素的含量
- Author:
Juan YANG
;
Jingqun HUANG
;
Hong LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Rujiexiao tablets;
Curcumine;
Content determination
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for the determination of the content of curcumine in Rujiexiao tablets.METHODS:The separation of sample was performed on VP-ODS C18 column (250 mm?4.6 mm,5 ?m).The mobile phase consisted of acetonitrile-0.2% phosphoric acid(61∶39) with a flow rate of 1.0 mL?min-1.The detection wavelength was set at 428 nm. RESULTS:The calibration curve of curcumine was linear within the range of 0.036～0.216 ?g (r=0.999 8) with an average recovery of 98.93%(RSD=0.99%,n=9).CONCLUSION:This method was proved to be simple,reliable and reproducible,and applicable for the quality control of Rujiexiao tablets.
