Assay Method for Sustained-Release Palmatine Tablet in Beagle Dog
- VernacularTitle:黄藤素缓释片在Beagle犬体内测定方法研究
- Author:
Zhaohui JIN
;
Xuehua JIANG
;
Ting XU
;
Yao TANG
- Publication Type:Journal Article
- Keywords:
Palmatine;
RP-HPLC;
Beagle dog;
Plasma concentration
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an assay method for Sustained-release palmatine tablet in beagle dog. METHODS:Sample separation was performed on Diamonsil ODS C18 column(250 mm?4.6 mm,5 ?m) with mobile phase consisted of acetonitrile-water (1∶1,containing NaH2PO4 3.4 g and SDS 1.7 g in every 1 000 mL water,pH was adjusted to 3 by H3PO4) with a flow rate of 1.0 mL?min-1.The column was kept at 35 ℃ and the detective wavelength was set at 342 nm.RESULTS:The linear serum concentration range was 0.005～0.5 ?g?mL-1(r=0.999 9) and the recovery rate was(102.22?2.48)%. CONCLUSION:The method was proved to be simple,accurate,sensitive and reproducible,and it was up to the standards for the study of pharmacokinetics and bioavailability of Sustained-release palmatine tablet in beagle dog.
