Determination of Capsaicin in Capsicum Annuum by RP-HPLC
- VernacularTitle:RP-HPLC法测定辣椒中辣椒碱的含量
- Author:
Daode HU
;
Lei GU
;
Huijuan YAO
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Capsicum annuum;
Capsaicin;
Content determination
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of capsaicin in Capsicum annuum. METHODS:The determination was performed on Zorbax Eclipse XDB-C18(150 mm?4.6 mm,5 ?m) column with column temperature set at 40 ℃.The mobile phase consisted of acetonitrile and 0.1% phosphate acid solution (45∶55,pH=2.6) with flow rate at 1.0 mL?min-1 and detective wavelength set at 281 nm.RESULTS:The calibration curve of capsaicin was linear over the concentration range of 30～70 mg?L-1(r=0.999 9).The average recovery for capsaicin was 101.33 %(RSD=1.57%,n=9).CONCLUSION:The method is simple,rapid and accurate,and suitable for the determination of capsaicin in Capsicum annuum.
