Determination of Dachnetin in Qufeng Xitong Plasters by HPLC
- VernacularTitle:HPLC法测定祛风息痛膏中瑞香素的含量
- Author:
Tao ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Qufeng xitong plaster;
Daphnetin;
Content determination
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method of the determination of dapchnetin in Qufeng xitong plaster. METHODS:The sample was determined on Sinochrom ODS-BP(250 mm?4.6 mm,5 ?m) column.The mobile phase consisted of acetonitrile-0.2% phosphoric acid(14∶86) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 327 nm and the sample size was 20 ?L.RESULTS:The calibration curve of daphnetin was linear over the concentration range of 20～120 ?g?mL-1(r=0.999 6).The average recovery was 99.36%(RSD=0.70%,n=6).CONCLUSION:The method was proved to be simple,rapid,accurate with satisfactory recovery rate,and it can be used for the quality control of Qufeng xitong plaster.
