Isolation of Calcium-Tolerant Cardiomyocytes from Rats
- VernacularTitle:钙耐受大鼠心肌细胞分离方法研究
- Author:
Jiqiang YU
;
Er GAO
;
Huirong HAN
;
Ning LI
- Publication Type:Journal Article
- Keywords:
Rat;
Calcium tolerant;
Myocardial cell;
Isolation;
Patch clamp
- From:
China Pharmacy
1991;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the method for rapid and effective isolating of calcium-tolerant cardiomyocytes(CTC) from rats. METHODS: CTC were obtained by retrograde perfusion using modified Langendorff heart perfusion apparatus. After incubation of cardiomyocytes in KB solution, the ion currents were recorded with patch-clamp technique in whole-cell mode. RESULTS: Of the whole isolated cells, 70% were survival. Of which, 80% were CTC. A typical outward potassium current was recorded. CONCLUSIONS: A large number of CTC with normal electrophysiological characteristics can be obtained by mastering the digestion time based on variation of perfusion pressure and keeping all the factors in cell isolation under control.
