Purification of the Anti-PMOP Effective Fraction from Camellia semiserrata Seed with DiaionHP-20 Macroporous Adsorption Resin
- VernacularTitle:DiaionHP-20富集纯化南山茶种子抗原发Ⅰ型骨质疏松有效部位的工艺研究
- Author:
Hongjing LIN
;
Xiaojuan WU
;
Qian TANG
;
Haifeng SHI
;
Ling TANG
;
Baomin FENG
;
Yongqi WANG
- Publication Type:Journal Article
- Keywords:
Camellia semiserrata;
DiaionHP-20 macroporous adsorption resin;
PMOP(primary Type I osteoporosis);
Ef-fective fraction;
Process condition
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the technical conditions and parameters of DiaionHP-20 macroporous adsorption resin for the purification of the anti-PMOP(primary Type I osteoporosis) active fraction from Camellia semiserrata seed.METHODS: With the elution ratios and purity of total flavonoids and polyphenol from Camellia semiserrata as indexes,the technical parameters of DiaionHP-20 macroporous adsorption resin including the adsorption quantity,the loading sample,the elution concentration and volume of alcohol were investigated.RESULTS: The adsorption quantity of DiaionHP-20 macroporous resin on total flavonoids and polyphenol were 59.5 mg?g-1 and 23.9 mg?g-1 respectively.The optimal technical conditions were as follows:the quantity of drug was 10.5 times that of resin;water-soluble foreign substance was washed off with distilled water weighing 3 times the weight of resin,and total flavonoids and polyphenol were eluted using 25% ethanol weighing 13.5 times the weight of resin.CONCLUSION:DiaionHP-20 macroporous resin had good purification effects on total flavonoids and polyphenol from Camellia semiserrata seeds.The process is simple,low cost,and suitable for lavge-scale production.
