Effects of Lingqi Capsule on the Proliferation of Human Pulmonary Adenocarcinoma A549 Cells in Vitro and the Expression of Apoptosis Related Genes-bcl-2 and Bax
- VernacularTitle:灵芪胶囊体外对人肺腺癌A549细胞增殖及凋亡相关基因表达的影响
- Author:
Honglin GANG
- Publication Type:Journal Article
- Keywords:
Lingqi capsule;
Serum-pharmacology;
Human pulmonary adenocarinoma A549 cell;
Proliferation;
Apoptosis;
Gene expression
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVE:To observe the effects of Lingqi capsule(LQC) on the proliferation of human pulmonary adenocarcinoma A549 cells in vitro and the expression of apoptosis related genes.METHODS: The inhibition rates of A549 cells treated with LQC-contained serum were detected with MTT assay.And RT-PCR was used to measure the expression on mRNA of apoptosis related genes-bcl-2 and bax of A549 cells.The morphological change of A549 cells in vitro were observed with Wright-Giemsa staining.RESULTS: The LQC-contained serum in various concentrations could inhibit the proliferation of A549 cells in a dose-dependent manner.The results of RT-PCR showed that LQC-contained serum could down-regulate the expression of bcl-2 gene but up-regulate the expression of bax gene.LQC-contained serum also could change the morphological structure of A549 cells.CONCLUSION: LQC-contained serum has a regulatory effect on apoptosis related genes,which might be one of the main mechanisms of its anti-tumor biological effects.
