Expression of ROCK Ⅰ and TGF-?_1 in pulmonary arterioles of rat with chronic thromboembolism pulmonary hypertension
- VernacularTitle:ROCKⅠ及TGF-?_1在慢性血栓栓塞性肺动脉高压大鼠肺小动脉的表达
- Author:
Mingxin CHEN
;
Yimei ZHANG
;
Wenjuan LIU
;
Yufei GAO
;
Liling WANG
- Publication Type:Journal Article
- Keywords:
Thromboembolism;
Pulmonary hypertension;
Rho kinase;
Transforming growth factor beta
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the dynamic expression of Rho kinase(ROCK I) and transforming growth factor ?1(TGF-?1) in pulmonary arterioles of rat with chronic thromboembolic pulmonary hypertension.METHODS: Sixty-four male Wister rats were randomly divided into eight groups: beginning control group,embolism for 3 d,1 week,2 weeks,4 weeks,8 weeks,12 weeks groups and end control group.The pulmonary thromboembolism(PTE) model was established by injecting thrombin into jugular vein two times in two weeks and each rat underwent peritoneal injection with tranexamic acid one time a day during experiment to prevent thrombolysis.The mean pulmonary artery pressure(mPAP),right ventricular hypertrophy index(RVHI),relative medial thickness of small pulmonary arteries(PAMT) and vessel wall area/total area(WA/TA) were measured.The levels of ROCK I mRNA and TGF-?1 protein in rat pulmonary artery were determined by in situ hybridization,immunohistochemistry and image analysis,respectively.RESULTS: mPAP,PAMT and WA/TA were higher respectively in embolism from 4 weeks group to 12 weeks group than those in beginning control group(mPAP: all P
