The Changes of SOD,MDA and Apoptosis in the Auditory Cortex,the Cochlear Nuclei and the Cochlea of the Rats with Age-Related Hearing Loss
- VernacularTitle:年龄相关性听力损失大鼠听皮层、蜗核、耳蜗SOD、MDA变化及神经细胞凋亡初步观察
- Author:
Yuan ZHOU
;
Dinghua XIE
;
Bin PENG
- Publication Type:Journal Article
- Keywords:
Age-related hearing loss;
Superoxide dismutase;
Malondialdehyde;
Apoptosis;
Flow cytometry
- From:
Journal of Audiology and Speech Pathology
1998;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of superoxide dismutase(SOD),malondialdehyde(MDA) and apoptosis in the auditory cortex,the cochlear nuclei and the cochlea of the rats with age-related hearing loss,and to understand the relationship between oxidative damage and apoptosis with presbyacusis.Methods Three-month-old rats(ABR 20?5 dB SPL) and eighteen-month-old rats(ABR 55?10 dB SPL) were used in the study.T-SOD activity was measured by xanthine oxidase(XO) method.The MDA level was examined by the thibabituric acid(TBA) method.Apoptosis was examined by Annexin V-FITC/PI staining with flow cytometry(FCM).Results The SOD activities decreased and the MDA levels increased significantly in the auditory cortex,the cochlear nuclei and the cochlea of the eighteen-month-old rats(P
