Uptake of Self-assembled Sodium Alginate Nanoparticles in Human Colon Carcinoma Caco-2 Cell in Vitro
- VernacularTitle:人结肠癌Caco-2细胞对自组装海藻酸钠纳米粒摄取的体外研究
- Author:
Yanli GAO
;
Sai LIU
;
Keke WANG
;
Nan YU
- Publication Type:Journal Article
- Keywords:
Caco-2 cell;
Self-assembled sodium alginate nanoparticles;
Uptake
- From:
China Pharmacy
1991;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the uptake of self-assembled sodium alginate(SA) nanoparticles labeled by FITC(sSAN-FITC) in Caco-2 cells and the influencing factors.METHODS: Fluorescence microscope was utilized to exam the uptake of sSAN-FITC in Caco-2 cells.Multifunction continuous spectrum enzyme-linked immunosorbent assay system was used for the detection of the fluorescence intensity of FITC in the cells and quantitative assay of the effect of sSAN concentration,its action time and pH on the uptake of sSAN-FITC in Caco-2 cells,with blank group as control.RESULTS: Pictures of Caco-2 cells taken by fluorescence microscope showed uptake of sSAN-FITC by Caco-2 cells.As compared with blank control group,among the 3 factors,the fluorescence intensity increased with the increase of the sSAN concentration and the prolonging of the action time(P
