Study on the Extracting and Purifying Process of Achyranthes Bidentata Polysaccharides
- VernacularTitle:牛膝多糖的提取纯化工艺研究
- Author:
Jianping HOU
- Publication Type:Journal Article
- Keywords:
Achyranthes bidentata polysaccharides;
Water decoction;
Deproteinization;
Desalination;
Alcohol precipitation
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the extracting and purifying process of Achyranthes bidentata polysaccharides(ABP). METHODS:Crude polysaccharide was obtained by extracting ABP raw material by water decocting method.The Crude polysaccharide was purified(deproteinization,desalination and alcohol precipitation) to obtain ABP.The content of total polysaccharide was taken as an evaluation index to optimize the optimum conditions of extracting and purifying.RESULTS: The optimum water decoction conditions were as follows:ABP raw material was extracted three times (2 h/time) by adding 10 times amount of water.As for the deproteinization process,TCA(trichloracetic acid) method was superior to Sevage method and tannic acid method;Desalination by molecular sieve was superior to that by dialysis method; and alcohol precipitation was performed with 80% alcohol.CONCLUSION:ABP can be prepared by water decoction,deproteinization,desalination and alcohol precipitation of its raw material.
