Validation of Determination Method of the Content of Morphine in Compound Glycyrrhiza Oral Solution
- VernacularTitle:复方甘草口服溶液中吗啡含量测定方法的验证
- Author:
Qingfen WANG
;
Shaozhong ZHENG
;
Mingqiong ZHANG
;
Rong ZHANG
- Publication Type:Journal Article
- Keywords:
Morphine;
Content determination;
Compound Glycyrrhiza oral solution;
Solid phase extraction-HPLC;
Validation
- From:
China Pharmacy
2007;0(36):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To validate the determination method of the content of Morphine in Compound Glycyrrhiza oral solution. METHODS: Samples were detected by solid phase extraction-HPLC on Kromasil C8 column (250 mm?4.6 mm,5 ?m) with mobile phase consisted of 0.05 mol?L-1 KH2PO4-0.0 025 mol?L-1 C14H15NaO3S?2H2O-acetonitrile using gradient elution.The detection wavelength was 220 nm and the flow rate was 1.0ml.min-1.RESULTS:The linear range of morphine was 2.02～20.2 ?g?mL-1(r=0.999 7),and the average recovery was 99.0%(RSD=0.40%,n=9).CONCLUSION: The method is accurate,sensitive and stable,and accurate and reliable in determination results.
