Effect of Kang Xianling decoction on TGF-?_1-Smad pathway in a unilateral ureteral obstruction rat model
- VernacularTitle:抗纤灵方对单侧输尿管梗阻大鼠TGF-?_1-Smad通路的影响
- Author:
Yumin LIU
;
Yue ZHANG
;
Liqun HE
;
Kejian LIU
;
Jing LI
;
Haiying LU
;
Zhifen HAN
;
Huifang PANG
;
Min LIN
- Publication Type:Journal Article
- Keywords:
Drugs,Chinese herbal;
Ureteral obstruction;
Transforming growth factor beta;
Protein Smad2
- From:
Chinese Journal of Pathophysiology
2000;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of Kang Xianling decoction,comprised of dahuang,danshen,taoren,niuxi and danggui,on TGF-?1-Smad pathway in unilateral ureteral obstruction rat model.METHODS: Eighteen male SD rats were divided into 3 groups,sham group,model group and model group treated with Kang Xianling decoction randomly.Renal interstitial fibrosis model was established in rats by unilateral ureteral obstruction(UUO).After treatment for additional 14 d,parameters of hydroxyproline in obstructed kidney from 3 groups were analyzed.Rats were sacrificed and the pathological statuses of their kidneys were checked by HE staining and electron microscopy.Transforming growth factor-?1(TGF-?1) mRNA in kidney tissue was determined by RT-PCR.TGF-?1 receptor Ⅰ(T?RⅠ),TGF-?1 receptorⅡ(T?RⅡ),phosphorylated Smad2 and Smad2 protein were determined by Western blotting.RESULTS: Parameters of hydroxyproline in animals of model group were significantly increased than those in sham operation group(P
