Study on the Fingerprints of Rheum palmatum Planted in Kangding Area
- VernacularTitle:康定地区人工种植掌叶大黄的HPLC指纹图谱研究
- Author:
Rong HUA
- Publication Type:Journal Article
- Keywords:
Rheum palmatum;
Kangding area;
Plant;
HPLC;
Fingerprint
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the HPLC fingerprints of Rheum palmatum planted in Kangding area.METHODS:The samples were separated on a Shim-pack ODS(150 mm?4.6 mm,5 ?m)with column temperature kept at 30 ℃;the mobile phase consisted of 0.1% H3PO4 methanol-0.1% H3PO4 aqueous solution by linear gradient elution with a flow rate of 1.0 mL?min-1 and detection wavelength of 280 nm.RESULTS:The HPLC fingerprints of Rheum palmatum planted in Kangding area had been established in which 56 common peaks were noted.Similarity of ten batches crude medicines was calculated,and a common template was set up.CONCLUSION:The established method is simple and feasible and by which the general quality of Rheum palmatum planted in Kangding area can be reflected.