Determination of Water-soluble Active Principles in Different Parts of Salvia miltiorrhiza by Capillary Zone Electrophoresis
- VernacularTitle:毛细管电泳法测定白花丹参不同部位水溶性有效成分
- Author:
Yingjie CUI
;
Yuqin LI
;
Nana YANG
;
Dawei LI
;
Xiaomin ZHAO
;
Zuoli XIA
- Publication Type:Journal Article
- Keywords:
Capillary zone electrophoresis;
Protocatechuic aldehyde(PAH);
Danshensu(DSS);
Protocatechuic acid(PA);
Salvia miltiorrhiza;
Content determination
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a high performenc capillary zone electrophoresis method for the determination of the water-soluble active ingredients in different parts of the Salvia miltiorrhiza.METHODS:Capillary electrophoresis was conducted using uncoated capillary column(75 ?m?50.2 cm,effective length=40 cm)with 5 mmol?L-1 phosphate-borax(ph7.4)as running buffer by pressure injection(0.5 psi/4 s)and constant voltage separation(20 kV)with a detection length of 210 nm and a column temperature of 25 ℃.RESULTS:A complete baseline separation of PAH,DSS and PA was achieved within 8 min under the optimized conditions.The good linear range was from 2.5 ?g?mL-1 to 200.0 ?g?mL-1 for all the three ingredients.The average recoveries of the 3 ingredients were 100.04%,99.99%,and 100.01%,respectively,with RSD at 1.75%,1.73%,and 1.74%,respectively.CONCLUSION:The method is satisfactory in precision,recovery and linearity,and it can be used to determine three water-soluble active ingredients in different parts of the Salvia miltiorrhiza.
