Simultaneous Determination of Berberin Hydrochloride,Baicalin and Glibenclamide in Kangtang Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法同时测定康糖片中盐酸小檗碱、黄芩苷、格列本脲的含量
- Author:
Xingong WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Kangtang tablet;
Berberin hydrochloride;
Baicalin;
Glibenclamide;
Content determination
- From:
China Pharmacy
2007;0(33):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an RP-HPLC method for simultaneous determination of Berberin hydrochloride,Baicalin and Glibenclamide in Kangtang tablets.METHODS:Separation of samples was achieved on a Diamond C18 analytical column(250 mm?4.6 mm,5 ?m).The mobile phase consisted of methanol-0.3% H3PO4(containing 0.3% triethylamine,pH2.5)using gradient program at a flow rate of 1.0 mL?min-1 and a detection wavelength of 230 nm.RESULTS:The linear ranges of Berberin hydrochloride,Baicalin and Glibenclamide were 0.02～1.0 mg?mL-1,0.02～1.0 mg?mL-1,0.000 8～0.04 mg?mL-1,respectively.The average recoveries were 100.16%,99.32% and 97.23%,respectively.The RSD were 1.97%、1.77%、1.28%,respectively(n=6).CONCLUSION:The method is simple,sensitive with high recovery rates and it is suitable for the quality control of Kangtang tablets.
