An experimental model of ischemia in rabbit hindlimb.
10.3346/jkms.2001.16.5.630
- Author:
Ju Hee HONG
1
;
Yong Whee BAHK
;
Jun Suk SUH
;
Byung Kook KWAK
;
Hyung Jin SHIM
;
Jin Soo KIM
;
Ho Sung KIM
;
Young Ho MOON
;
Sung Jin KIM
;
Jin Wook CHUNG
;
Jae Hyung PARK
Author Information
1. Department of Radiology, Sungae General Hospital, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Ischemia;
Hindlimb;
Rabbits
- MeSH:
Angiography;
Animal;
Blood Pressure;
*Disease Models, Animal;
Hindlimb/*blood supply;
Ischemia/*physiopathology/radiography;
Male;
Rabbits
- From:Journal of Korean Medical Science
2001;16(5):630-635
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to establish an experimental model of ischemia for the investigation of new treatment modality of limb-threatening ischemia. We produced ischemia in the hindlimbs of 8 New Zealand white rabbits. Under general anesthesia, the left femoral artery was exposed, freed, and excised from distal external iliac artery to proximal popliteal and saphenous arteries. And then both hindlimbs were serially examined to assess the ischemia according to the time table until postoperative 6 weeks. We assessed clinical observation, blood pressure, radioisotopic perfusion scan, and angiography. Clinical ischemic changes of the operated feet were observed in 63%. The blood pressure of left calves was measurable on postoperative day 3 (p<0.05, vs preoperative day 2) and then gradually increased to reach a plateau in postoperative week 6. Radioisotopic arterial perfusion showed similar profiles as in blood pressure. Angiography of ischemic hindlimbs demonstrated a few collateral vessels arising from the internal iliac artery with the reconstitution of the posterior tibial artery in postoperative week 2. In postoperative week 6, collaterals remained the same in number. However, these became dilated and tortuous and showed reconstitution in distal hindleg. In conclusion, this is a reproducible, measurable, and economical animal model of hind limb ischemia.
