The protective role of erythropoietin induced by hypoxic preconditioning on cerebral injury and cognition after global cerebral ischemia
- VernacularTitle:低氧预处理诱导促红细胞生成素对脑缺血后损伤及认知功能的保护作用
- Author:
Zhenhong WANG
;
Xiangrui WANG
- Publication Type:Journal Article
- Keywords:
global ischemia/reperfusion injury;
hypoxic preconditioning;
cognition;
cellular necrosis;
erythropoietin
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effects of hypoxic preconditioning(HPC) on the improvement of the cognitive dysfunction(learning and memory) and the damage in hippocampus induced by global cerebral ischemia/reperfusion(I/R) in CA1 and CA3 for 5 days in rats,and on the regulation of expression of erythropoietin(EPO) protein to approach the mechanism of the protection.Methods One hundred and twenty adult male Sprague-Dawley(SD) rats were divided into four groups randomly: sham group,I/R group,HPC24 group(hypoxia for 24 hours before I/R) and HPC48 group(hypoxia for 48 hours before I/R).Hang(motor function),passive avoidance and Morris water maze tests were carried out on the 5th day after I/R to measure the motor and cognition functions;hematoxylin-eosin(HE) staining was used to detected histopathological changes in hippocampus tissues;and the contents of EPO were tested by immunohistochemistry at 1 hour and 4 hours after I/R from hippocampus CA1 and CA3 regions.Results Hang,passive avoidance and Morris water maze tests showed that I/R can injure rat cognition;the improvement of cognition was marked in HPC groups, and it was shown that the effects were more significant in HPC48 group than those in the HPC24 group(P
