Effects of extract of ginkgo biloba on human tubular epithelial-mesenchymal transition induced by transforming growth factor-?_1
- VernacularTitle:银杏叶提取物对转化生长因子?_1诱导的人肾小管上皮细胞转分化的影响及其机制
- Author:
Xizhen XU
;
Changlong ZHENG
;
Ling TU
;
Gang ZHAO
;
Daowen WANG
- Publication Type:Journal Article
- Keywords:
Extract of ginkgo biloba;
Transforming growth factor beta;
Renal tubular epithelial cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:to investigate the effects of extract of ginkgo biloba (EGB) on human tubular epithelial-mesenchymal transition induced by transforming growth factor-?1.METHODS: HK2 cells were induced to epithelial-mesenchymal transition by transforming growth factor-?1 (TGF-?1, 10 ?g/L). EGB was added into the medium of HK2 cells 2 h before TGF-?1 was added. The expressions of E-cadherin, ?-smooth muscle actin (?-SMA), NADPH oxidase p67phox and superoxide dismutase (SOD) were determined by Western blotting. Malondialdehyde (MDA) in the mediums of HK2 cells was detected. RESULTS: EGB significantly attenuated the downregulation of E-cadherin, the upregulation of ?-SMA and p67phox, the downregulation of SOD and the upregulation of MDA in HK2 cells induced by TGF-?1.CONCLUSION: EGB significantly attenuates human tubular epithelial-mesenchymal transition induced by TGF-?1, and its underlying mechanism is that EGB attenuates the upregulation of p67phox and the downregulation of SOD induced by TGF-?1.
