Construction of fusion expression vector AR-GFP and its expression in Hek293 cells
- VernacularTitle:AR-GFP融合基因真核表达载体的构建及在HEK293细胞中的表达
- Author:
Jianwei LIU
;
Ling YE
;
Jing LIU
;
Dongbin NIU
;
Mingmei DU
;
Yuhong GAO
- Publication Type:Journal Article
- Keywords:
Aldose reductase;
Green fluorescent protein;
Gene expression;
Hek293 cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To construct a hAR and GFP fusion gene vector and to observe the AR-GFP gene expression in Hek293 cells. METHODS: A recombined vector pcDNA3.1/myc-HisA-AR-GFP (pH-AG) was constructed by gene engineering technique. The recombined vector was transfected into Hek293 cells using calcium phosphate. RESULTS: AR-GFP fusion protein was successfully expressed in Hek293 cells without biologic activity, which was confirmed by fluorescence microscopy and Western blotting. CONCLUSION: The Hek293 cells transfected by AR-GFP fusion gene can express its protein successfully. However, it is not a cellular model for ARI screening.
