Effects of adiponectin siRNA on the glucose transport in 3T3-L1 adipocytes
- VernacularTitle:脂联素siRNA对3T3-L1细胞基础葡萄糖转运的影响
- Author:
Fanping MENG
;
Changben WANG
;
Liangqiong LI
;
Po HAO
- Publication Type:Journal Article
- Keywords:
RNA interference;
Adenovirus vector;
Adiponectin;
Glucose transport;
3T3-L1 cells
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To construct the adenovirus vector with adiponectin (Acrp30) siRNA, and to observe its effect on the Acrp30 expression and glucose transport in 3T3-L1 adipocytes. METHODS: Mouse Acrp30 siRNA fragment was designed, synthesized and cloned into the adenovirus vector. 3T3-L1 cells were infected with the two recombinant adenoviruses, respectively. The mRNA expression and protein levels of Acrp30 in these cells were evaluated by RT-PCR and ELISA. Glucose transport was measured by 2-Deoxy-[3H]-D-glucose incorporation method. RESULTS: The recombinant adenoviruses were successfully constructed. They remarkably downregulated the expression of Acrp30 at both mRNA and protein levels in 3T3-L1 cells, and decreased the glucose transport in 3T3-L1 adipocytes (P
