Protective effects of nmhaFGF on NRK52E cell apoptosis induced by H_2O_2
- VernacularTitle:nmhaFGF对过氧化氢诱导的NRK52E细胞凋亡的拮抗作用
- Author:
Guangfan HAI
;
Hua XU
;
Jing YU
;
Zhijian SU
;
Qing ZHENG
;
Hong XU
- Publication Type:Journal Article
- Keywords:
Hydrogen peroxide;
Renal tubular epithelial cells;
Fibroblast growth factor 1;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The present study was designed to establish H2O2-induced NRK52E cell apoptotic model and to investigate the effects and the mechanism of nmhaFGF on the NRK52E cell apoptosis induced by H2O2. METHODS: In vitro experiment, a apoptotic model of normal rat kidney epithelium (NRK52E) was made by MTT method, Hoechst33342 dyeing and flow cytosorting (FCR). NRK52E cells were cultured with different concentrations of nmhaFGF and haFGF for 24 h before H2O2 was added. The apoptotic rate was detected with FCR method. RESULTS: H2O2 at concentration of 0.4 mmol/L, the optimal condition to establish the apoptotic model, was used to treat NRK52E cells for 18h. Different doses of nmhaFGF (0.01, 0.03, 0.10, 0.30, 1.00 mg/L) reduced the apoptotic rates with the dose rising. However, the decreasing tends of apoptotic rates with dose increasing for haFGF was not so obvious. CONCLUSION: nmhaFGF protects the NRK52E cells against apoptosis. The mechanism might be connected with its non-mitogenic property.
