Immunoprotection of Recombinant Elongation Factor-1 Against Echinococcus Granulosus in Mice
- VernacularTitle:细粒棘球蚴延伸因子-1重组蛋白对小鼠的免疫保护性研究
- Author:
Jian WANG
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Elongation factor;
Recombination protein;
Immunoprotection
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the protective immunity in mice immunized with rEg.EF-1 and its potential as vaccine candidate.METHODS: ICR mice were subcutaneously immunized with rEg.EF-1 for three times(with an interval of 2 weeks) and challenged with Eg 2 weeks after the completion the immunization,sacrificed at 20 weeks after infection with Eg.The rate of reduced hydatid cyst and the levels of IgE,IgG and IgG subclasses in sera were examined by enzyme linked immunosorbent assay,with adjuvant group as control.RESULTS: As compared with adjuvant group,the immunoprotection ability(rate of reduced hydatid cyst) in rEg.EF-1 group was 85.6%;The levels of IgG,IgG1 and IgG2a in mice immunized with rEg.EF-1 significantly increased(P0.05),and no significant difference was noted in IgG3 and IgE levels.CONCLUSIONS: The rEg.EF-1 antigen could induce certain immunoprotection,suggesting the potential of rEg.EF-1 as an vaccine candidate.
