Quantitative Method for the Bacterial Endotoxin in Aminoglycoside Antibiotics for Injection
- VernacularTitle:注射用氨基糖苷类抗生素细菌内毒素定量法的研究
- Author:
Zhijun HUANG
;
Haiyan LAO
;
Qiuxiao LIN
- Publication Type:Journal Article
- Keywords:
Kinetic turbidimetric limulus test;
Aminoglycosides antibiotics;
Bacterial endotoxin;
Interference test
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for endotoxin assay in aminoglycoside antibiotics for injection.METHODS: Kinetic turbidimetric limulus test was used for endotoxin assay in aminoglycoside antibiotics for injection.The recovery rate of bacterial endotoxin was determined though pre-interference experiments in which quantitative endotoxin was added to different concentrations of aminoglycosides with the concentration optimized,then an interference test on 4 different samples was conducted.RESULTS: At a concentration of 0.5 mg?mL-1,aminoglycosides didn't interfere with the limulus test if the amount of standard endotoxin was at 5.000,0.500,and 0.050 EU?mL-1,respectively.CONCLUSIONS: The kinetic turbidimetric limulus test could be used for endotoxin assay in aminoglycoside antibiotics for injection.
