Determination of the Relevant Substances in Tegafur Injection by HPLC
- VernacularTitle:HPLC法测定替加氟注射液的有关物质
- Author:
Yu CAO
;
Xiarong SUN
;
Qing ZHANG
- Publication Type:Journal Article
- Keywords:
TLC;
HPLC;
Tegafur;
Relevant substances
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of the relevant substances in tegafur injection.METHODS: The determination was performed with a Kromasil C18 column with the column temperature maintained at 30 ℃,and the mobile phase was methanol-water(25∶75) at a flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 282 nm and the sample size was 20 ?L.RESULTS: The relevant substances were well-separated from the chief agent,and the contents of the relevant substances in the sample were 2.35%,2.37%,and 2.24%,respectively.CONCLUSION: Compared with TLC,HPLC is more accurate and sensitive,and it is applicable for the determination of the relevant substances in tegafur injection.
