Simultaneous Determination of Sulfadiazine and Dexamethasone Acetate in Compound Nasal Ointment by HPLC
- VernacularTitle:HPLC法同时测定复方鼻膏中2组分的含量
- Author:
Chenfang LIANG
;
Yazhou WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound nasal ointment;
Sulfadiazine;
Dexamethasone acetate
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for simultaneous determination of the content of sulfadiazine and dexamethasone acetate in compound nasal ointment.METHODS: The determination was performed on Inertsil C8-3 column with column temperature set at 40 ℃;the mobile phase consisted of methanol-acetonitrile-water(72∶3∶25) with a flow rate of 1.0 mL?min-1.The detective wavelength was set at 240 nm and the sample size was 20 ?L.RESULTS: The linear ranges for sulfadiazine and dexamethasone acetate were 74.65～746.5(r=0.999 9) and 1.495～14.95 ?g?mL-1(r=0.999 7),respectively.The average recoveries were 100.72%(RSD=1.7%) and 100.41%(RSD=1.6%),respectively.CONCLUSION: This method is rapid,simple,accurate and reliable and suitable for the determination of the content of sulfadiazine and dexamethasone acetate in compound nasal ointment.
