Determination of Phenylhydrazine Residues in Edaravone by HPLC
- VernacularTitle:HPLC法测定依达拉奉中苯肼的残留量
- Author:
Jinlin LI
;
Guizhen FANG
;
Jiudi LIU
;
Lijuan KANG
;
Xiaojuan ZHOU
- Publication Type:Journal Article
- Keywords:
HPLC;
Edaravone;
Phenyldrazine;
Residues
- From:
China Pharmacy
2007;0(31):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of phenylhydrazine residues in edaravone.METHODS: The determination was performed on Diamonsil C18 column with 0.05 mol?L-1 ammonium acetate-acetonitrile(80∶20) as mobile phase at a flow of 1.0 mL?min-1.The wavelength of UV detector was set at 233 nm and the sample size was 20 ?L.RESULTS: The linear range of phenylhydrazine was 22～550 ng?mL-1(r=0.999 9);The average recovery was 98.10%(RSD=0.85%),and the detection limit was 44 pg.CONCLUSION: The analytical method is simple,reliable and sensitive,and suitable for the determination of phenylhydrazine residues in edaravone preparation.
