Study on HPLC Fingerprint of Punica granatum
- VernacularTitle:石榴皮药材HPLC指纹图谱研究
- Author:
Benhong ZHOU
;
Jie ZHANG
- Publication Type:Journal Article
- Keywords:
Punica granatum;
Fingerprint;
HPLC
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the chromatographic fingerprints of Punica granatum by HPLC and to study the quality of the granati cortex from different habitats.METHODS: The chromatographic column was kromasil C18 and the mobile phase was acetonitrile-H3PO4 buffer salt(containing 0.1% triethylamine)-water at a flow rate of 1 mL?min-1.The column temperature was maintained at 25 ℃and the detection wavelength was set at 240 nm.The fingerprints of different samples were compared with similarity evaluation software(2004 A) published by committee of codex.RESULTS:Twelve peaks were identified as the characteristic fingerprints of Punica granatum.All samples tested contained the same 12 peaks,but the content of the each peak showed difference among the samples,which resulted in the difference in their chromatograms.And the similarity was correlated with the habitat of the herb.CONCLUSION: This method shows high precision and good repeatability,and provides a reference standard for the quality control of Punica granatum.
