Determination of Oleanolic Acid and Ursolic Acid in Hedyotis corymbosa by Capillary Zone Electrophoresis with High Frequency Conductivity Detection
- VernacularTitle:毛细管电泳高频电导法测定水线草中齐墩果酸和熊果酸的含量
- Author:
Peiyi ZHU
;
Fenyun SONG
- Publication Type:Journal Article
- Keywords:
Capillary zone electrophoresis;
High frequency conductivity detection;
Hedyotis corymbosa;
Oleanolic acid;
Ursolic acid;
Content determination
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of Oleanolic acid and Ursolic acid isomers in Hedyotis corymbosa.METHODS: Capillary zone electrophoresis with high frequency conductivity detection was adopted.The separation of sample was performed on an uncoated fused silica capillary(55 cm?75 ?m ID) at an effective length of 50 cm.1.2 mmol?L-1 Triethylamine(TEA)-HCl(pH10.0) buffer containing 0.24 mmol?L-1 ?-cyclodextrin was selected for the running buffer solution.The voltage applied was 12.5 kV and the sample was injected by gravity for 10 s at a height of 20 cm.Brufen was used as internal standard.RESULTS: The linear ranges for Oleanolic acid and Ursolic acid were 3.9～39.0 ?g?mL-1(r=0.999 1) and 20.0～140.0 ?g?mL-1(r=0.999 0),respectively,and their average recovery rates were 96.5% and 96.0% respectively and RSD were 1.98% and 1.09%,respectively(n=6).CONCLUSION:It was proved that the method was simple,accurate,rapid and reproducible,and applicable for the quality control of Hedyotis corymbosa.
