Determination of Emodin in Fugan Oral Liquid by RP-HPLC
- VernacularTitle:RP-HPLC法测定复肝口服液中大黄素的含量
- Author:
Li AO
;
Qian ZHAO
;
Jian SONG
- Publication Type:Journal Article
- Keywords:
Emodin;
Fugan oral liquid;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for the determination of the content of Emodin in Fugan oral liquid.METHODS: The sample was separated on Zorbax SB-C18 column(150 mm?4.6 mm,5 ?m) with methanol-0.1%phosphoric acid solution(85∶15) as the mobile phase at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 430 nm and the column temperature was 30 ℃.RESULTS: The calibration curve was linear at a range of 0.075 2 mg～0.376 0 mg(r=0.999 96) for the Emodin.The average recovery was 99.77% and the related standard deviation(RSD) was 1.03%(n=6).CONCLUSION: This method was proved to be simple,quick and reproducible and can be used for the quality control of Fugan oral liquid.
