Determination of Nucleosides in Cordyceps sinensis Preparation by Dual-wavelength TLC-scanning
- VernacularTitle:双波长薄层扫描法测定虫草类制剂中核苷类化合物的含量
- Author:
Yan MA
;
Yu WANG
- Publication Type:Journal Article
- Keywords:
Cordyceps sinensis;
Jinshuibao capsule;
Zhiling capsule;
Ningxinbao capsule;
Dualwavelength TLC-scanning method;
Nucleosides;
Content determination
- From:
China Pharmacy
2007;0(30):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a dualwavelength TLC-scanning method for the determination of nucleosides in the preparation of Cordyceps sinensis.METHODS: The sample was analyzed on silica GF254 thin layer plate using 1% CMC-Na as adhesive and chloroform-ethylacetate-isopropanol-water-ammonia(8∶2∶6∶0.5∶0.12) as developing agent and detected by dualwavelength reflection sawtooth scanning(?s=254 nm and ?R=300 nm).RESULTS:Adenosine,Uracil and Uridnine had good linearity in the ranges of 11.16～58.03 ?g(r= 0.998 5),1.04～6.24 ?g(r=0.991 5) and 0.8～4.8 ?g(r=0.991 2) respectively,and their average recovery rates were 95.559%,96.366% and 95.270% and RSD were 0.74%,0.73% and 0.98% respectively(n=6).CONCLUSION: The method is simple,accurate,reproducible,and can be used as the quality control of Cordyceps sinensis preparation.
