The analytical method of three phenoxyalkanoic acid herbicides in grape leaf by dichloropropyl esterification and gas chromatography
- VernacularTitle:二氯丙基酯化-气相色谱法分析葡萄叶中3种除草剂
- Author:
Guobin XIN
;
Jiayi TAN
;
Lijuan YAO
- Publication Type:Journal Article
- Keywords:
Forensic toxicological analisis;
Phenoxyalkanoic acid herbicides;
Plant leaf analysis;
Carboxyl derivatization;
Gas chromatography
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo develop a analytical method of three phenoxyalkanoic acid herbicides,MCPA,2,4-D and dichlorprop,in grape leaf using dichloropropyl esterification and gas chromatography with electron-capture detection.MethodsSamples were pounded to pieces,added into internal standard 2,4-dichlorophenylacetic acid,acidified and extracted with ethyl ether.The extracts were cleaned up by liquid-liquid partition.The cleaned organic solutions were evaporated and the residues were dichloropropylated using sulfuric acid and 1,3-dichloropropan-2-ol.The formed esters were analysed by gas chromatography.ResultsThe detection limits of MCPA,2,4-D and dichlorprop in grape leaf were 20ng/g,20ng/g,and 30ng/g,respectively.The concentritions of herbicides in grape leaf were linear over the ranger 100~1000ng/ml.The average recoveries of MCPA,2,4-D and dichlorprop in spiked grape leaf at concentrition of 200ng/g were 98.8%,101.3% and 103.1% with the coefficients of variation 11.4%,11.0% and 9.3% respectively.ConclusionThis method is sensitive and accurate for determination of these three herbicides in poisoned grape leaf.
