A study on the content changes of tubulin in liver,spleen and kidney of rats killed by bleeding
- VernacularTitle:失血性休克大鼠肝肾脾细胞微管蛋白死后降解的检测
- Author:
Yang LIU
;
Jinxia KUAI
;
Yanwei ZHANG
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Postmortem interval;
Tubulin;
Western-blot
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the degradation of tubulin in liver,spleen and kidney of rats killed by bleeding and to explore its usefulness in estimation of postmortem interval(PMI).Methods 24 young male Sprague-Dawley rats were killed by bleeding and kept in an artificial climate incubator for 0,1,2,3,5 and 7d respectively,then the content of tubulin in spleen,liver,and kidney were examined by western-blot assay and the data of detection were analyzed statistically by using SPSS 11.5 software.Results Within a certain postmortem interval(PMI