The exploration of STR genotyping of minute cells by MiniFiler kit
- VernacularTitle:MiniFiler试剂盒进行微量细胞STR分型可行性探讨
- Author:
Qin SU
;
Hong YAN
;
Caixia LI
- Publication Type:Journal Article
- Keywords:
Forensic biological evidence;
Micromanipulation method;
MiniFiler kit;
Oral epithelial cells;
STR
- From:
Chinese Journal of Forensic Medicine
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Explore the feasibility of minute cells STR typing.Methods Minute cells which were obtained by micromanipulation method were amplified with MiniFiler kit,and detected by ABI 3130 genetic analyzer.Results Ten cells can be successfully genotyped.Complete genotyping results can be obtained for one,three or five cells but with stochastic effect.Conclusion Instability is observed in minute cells genotyping,so it can not be used in actual case work.Maybe increase the quantity of DNA template can improve the success rate.
