Matrix metalloproteinase-9 and cystoskeleton actin are involved in the increased permeability induced by hypoxia/ischemia status in a blood-brain barrier model
- VernacularTitle:MMP-9和细胞骨架肌动蛋白参与缺氧缺血诱导的体外血脑屏障模型通透性增加
- Author:
Na GAN
;
Fei YIN
;
Jing PENG
;
Weidong WANG
- Publication Type:Journal Article
- Keywords:
Blood-brain barrier;
Actin;
Matrix metalloproteinase-9;
Permeability
- From:
Chinese Journal of Pathophysiology
2000;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To understand the effects and approach the mechanisms of matrix metalloproteinase-9(MMP-9)and cystoskeleton actin on the permeability increasing of blood-brain barrier(BBB)model which was induced by hypoxia/ischemia status in vitro.METHODS:The BBB model was build by the co-culture of cell ECV304 and astrocytes in vitro,then divided randomly into control group,hypoxia/ischemia group and BB-1101 pretreatment group.The permeability of BBB was determined by [125I]-BSA.The expression and the disposition of actin were detected by direct-immunofluorescence and Western blotting.BB-1101,the MMPs inhibitor,was used to investigate if MMP-9 participate the process of the increasing of BBB models' permeability in hypoxia/ischemia status.RESULTS:Post-stimulation of hypoxia/ischemia for 5 h,the permeability of [125I]-BSA and amount expression of MMP-9 in hypoxia-ischemia group was increased compared with control group(P
